Bayanan samfurin
A matsayin masu biyo baya na sanannen Centrifuge 5424/5424 R, waɗannan sabbin samfuran biyu sun haɗa da duk samfuran da kuka fi so a cikin samfuran da suka gabata, tare da sabon rotor da fasalin software don yin aikinku na yau da kullun ya fi inganci da daɗi.
A musamman zane na iska sanyaya Centrifuge 5425 tabbatar da musamman shiru aiki don samar da m aiki yanayi. Sautin da aka tsara yana da ƙarancin sauti har zuwa mintuna 12 tare da matsakaicin amo na 45 dB (A). Wadannan gajeren lokacin gudu sun isa don kammala matakai na centrifugal ga yawancin kayan tsarkakewar DNA / RNA da aka saba amfani da su. Lokacin da aka yi aiki na dogon lokaci, mai sanyaya fan yana sanyaya zafi yadda ya kamata a cikin sauran aiki.
An inganta tsarin sanyaya na Centrifuge 5425 R yana tabbatar da mafi kyawun sakamakon rabuwa, wanda zai iya sanyaya a 4 ° C ko da a cikin iyakar saurin juyawa na rotor. A cikin kawai 8 mintuna pre-sanyi lokaci (~ 21 ° C zuwa 4 ° C), centrifuge zai iya aiki a kowane lokaci.
6 daban-daban rotors yanzu rufe wani m kewayon aikace-aikace, ciki har da aikace-aikacen kwayoyin halitta kamar DNA / RNA ko furotin tsarkakewa a cikin 1.5 ko 2.0 mL centrifuge ko centrifugal ginshike, kwayoyin cuta ruwa, girma yisti da kwayoyin halitta a cikin 5 mL tubing, da kuma trace gwaje-gwaje kamar PCR pre-sarrafawa / post-sarrafawa da qPCR tsarin gina a cikin 0.2 mL tubing, 8-rami allon ko raba 96 rami allon.
Eppendorf Kit rotor®
(FA-18x2-Kit) don 18 x centrifugal ginshike da 1.5/2.0 ml centrifugal bututun (max 18,565 x g)
Wannan rotor na musamman yana magance matsalolin da aka saba samu yayin amfani da MiniPrep Rotary Kit: An yanke rufin kwantena mai buɗewa a matakan wanke. Yawancin dalilin wannan matsalar shine cewa rotor na gargajiya ba zai iya ba da isasshen tallafi ga murfin bututun da aka buɗe yayin tsarin centrifugation ba. Eppendorf Kit Rotor yana da gefen da ya fi girma kuma a lokacin da aka tsara centrifugation, an sanya rufin kwantena a kan shi don haka ba za a yanke su ba. Daga yanzu, murfin kwantena zai kasance a wurinsa.
Rotor FA-10x5 don bututun Eppendorf na 10 x 5.0 mL (har zuwa 21,300 x g)
a cikin Eppendorf Tubes®An girbe kwayoyin cuta a cikin 5.0 mL, ana noma yisti ko ƙwayoyin halitta, da kuma tsarkakewar DNA / RNA da DNA plasmid, amfani da wannan rotor ya zama mai sauki. Hakanan an ba da adafta don yin amfani da bututun centrifugal na 1.5/2 ml tare da bututun centrifugal na HPLC da Cryo ba tare da maye gurbin mai juyawa ba.
