Tsarin nazarin hoto na algae

Algae shine babban sunan ƙofar algae mai shuɗi, ƙofar algae mai ido, ƙofar algae mai zinariya, ƙofar algae mai launin kore, ƙofar algae mai launin ruwa, ƙofar algae mai launin ruwa da sauransu. Ana da nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau Algae, a matsayin mafi mahimmancin masu samar da ruwa, suna taka muhimmiyar rawa ga tsarin halittu gaba ɗaya har ma da kwanciyar hankali na duniya. A lokaci guda, algae da yawa na tattalin arziki suna taka muhimmiyar rawa a cikin masana'antun abinci, magunguna, makamashi da sauransu. Kuma abubuwan da ke haifar da muhalli masu cutarwa kamar ruwa, ruwan ruwa, da kuma algae.

A cikin binciken algae, ana buƙatar cikakken nazarin halayen algae, musamman ma'auni da nazarin photosynthetic physiology, morphology, launi, tsari da rarraba launuka, gudummawar photosynthesis na launuka daban-daban, tilasta physiology da sauransu, don yin ganin algae phenotypes, physiological muhalli da aiki. Wannan yana buƙatar tsarin daukar hoto na phenotype wanda aka tsara musamman don nau'ikan algae.

Tsarin nazarin zane-zane na algae yana kunshe da FMT150 algae culture da online monitoring unit, FluorCam fluorescence ko multi-spectrum fluorescence imaging unit, FKM multi-spectrum fluorescence dynamic microimaging unit, high spectrum imaging unit (ko multi-spectrum imaging unit), RGB launi imaging unit, da dai sauransu, shi ne mafi sassauƙa, mafi cikakken aiki, mafi inganci, fasaha-jagorancin tsarin nazarin zane-zane na algae da binciken muhallin halitta.

Binciken daukar hoto na phenotype na coral co-organisms, hagu: Binciken daukar hoto na coral shrinkage-fadada ba tare da lokaci guda ba NDVI multi-spectral imaging tare da GFP green fluorescent protein; hoton dama; Samfurin farfajiyar murjani da samfurin tsaye don hoton RGB, hoton fluorescence na chlorophyll da nazarin hoton NDVI mai yawa a ƙarƙashin yanayin daidaitawa da haske mai ƙarfi (Leal MC,da sauransu.2015）

Main fasali Features da fasaha nuna alama:

1. Tsarin FMT150Algae noma da online sa ido fasaha: musamman hadewa bioreactor da sa ido kayan aiki don modular daidai haske noma da kuma physiological sa ido na ruwa mai kyau, algae na teku da algae mai shuɗi, da dai sauransu:

1) Size Volume Zaɓi: 400ml, 1L, 3L, da dai sauransu, za a iya tsara manyan al'adu kwantena na 25L, 120L

2) Full LED haske tushen: ja haske, blue haske ko farin haske, ja haske biyu launi haske tushen, sauran haske inganci za a iya tsara, max haske karfi har zuwa 3000 μmol (photons).m^-2sa^-1

3) Daidai sarrafa zafin jiki, haske karfi, noma sake zagayowar, CO₂Taro da sauran al'adu yanayi, kwaikwayon halitta sake zagayowar canje-canje, kuma za a iya yi steady ko steady turbid al'adu

4) Online real-lokaci sa ido da zafin jiki, pH, narkewa oxygen, O₂/ KA₂Daban-daban muhalli da kuma physiological sigogi kamar gudanarwa, abinci mai gina jiki gishiri canje-canje, haske yawa, chlorophyll fluorescence sigogi (nuna tilasta yanayin da kuma physiological yanayin)

5) Aiki waje iko, data real-lokaci online sa ido da kuma adana ta hanyar sadaukar da kwamfuta software

6) Zaɓi tare da MC1000 8 tashar algae culture tare da online monitoring unit don sauri maimaita noma gwaji

Rhineland Chloria polyomic analysis, hagu: daidai sarrafa noma ta amfani da FMT150; Hoton dama: Binciken fluorescence na chlorophyll ta amfani da FluorCam (Strenkert, 2019, PNAS)

2. FluorCamChlorophyll fluorescence imaging fasaha: Za a iya zaɓar daban-daban samfuran da kuma hoto girman tsarin hoto na Chlorophyll fluorescence bisa ga buƙatu, ko zaɓi kamar GFP / YFP mai daidaitaccen yanayin hoto, PAR sha / NDVI hoto da sauransu:

1) Mai ɗaukar hoto na chlorophyll fluorescence, yankin hoto na 31.5x41.5mm, ana iya amfani da shi a filin daji da na dakin gwaje-gwaje don nazarin hoto na chlorophyll fluorescence, kuma ana iya amfani da shi a cikin tsarin photosynthesis don ma'auni na photosynthesis da ma'auni na nazarin hoto na chlorophyll fluorescence

2) Rufe-irin (one-in-one) chlorophyll fluorescence imaging tsarin, mafi aiki (tare da duk Protocols ciki har da OJIP, QA re-oxidation dynamics, da dai sauransu), mafi inganci rabo, amfani da mafi dacewa tebur shuka phenotype imaging bincike kayan aiki, za a iya a lokaci guda na shuka PAR sha, spectrum reflection index NDVI, da dai sauransu don yin imaging ma'auni

3) FluorCamModular Chlorophyll fluorescence imaging tsarin, tare da 13x13cm daidaitaccen version da 20x20 babban version, modular tsarin, tare da scalability, zaɓi tare da farin LED haske tushen (don kwaikwayon halitta haske tushen), shuɗi LED haske tushen, kore LED haske tushen, ja LED haske tushen, shuɗi LED haske tushen da sauransu daban-daban motsa haske tushen don haske gwaji, don motsa daban-daban algae photosynthetic pigment furotin (da ke ƙasa ne mai ɗaukar hoto, one-in-one, modular Chlorophyll fluorescence imaging tsarin)

4) FluorCamBabban dandalin hoto na chlorophyll fluorescence tare da yankin hoto har zuwa 35x35cm(Hoton da ke ƙasa ne mai ɗaukar hoto, mai ɗaukar hoto, tsarin daukar hoto na chlorophyll fluorescence daBabban dandalin daukar hoto na chlorophyll fluorescence）

5) FluorCamMulti-spectral fluorescence imaging fasahar tsari, sanye da UV UV haske da kuma na musamman tace motsawa da kuma gano multi-spectral fluorescence na algae, musamman dace da biyu metabolism da kuma cututtuka phenotype bincike. Akwai wani-a-daya (hoto yankin 13x13cm) ko modular (hoto yankin 13x13cm ko 20x20cm) don zaɓi, kuma akwai zaɓi tare da dakin gwaje-gwaje babban multi-spectrum fluorescence hoto dandamali

Jami'ar Ocean ta China ta Amfani da FluorCamMulti-spectrum fluorescence imaging tsarin bincike striped purple vegetablesPyropia yezoensisAmsar metabolism na biyu bayan kamuwa da cutar Red Corruption (Tang L, 2019)

3. FKMMulti-spectral fluorescence Dynamic Microimaging Technology: Fasahar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta hanyar daukar hoto ta han Ya ƙunshi ƙarfafa microscope wanda ya ƙunshi m sassa, high ƙuduri CCD kyamara, motsawa haske tushen rukuni, spectrometer, zafin jiki sarrafawa module da kuma dacewa sarrafawa naúrar da kuma keɓaɓɓun aiki tashar da kuma bincike software. Ba wai kawai yana iya yin nazarin chlorophyll fluorescence da multi-spectrum fluorescence imaging na microalgae, ƙwayoyin halitta guda ɗaya, chlorophyll guda ɗaya, har ma da sassan cystic na substrate-substrate ba, har ma yana iya yin nazarin hoto na furotin fluorescent, launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin

1) An gina dukan tsari na yanzu chlorophyll fluorescence bincike, kamar Fv / Fm, Kautsky induced tasiri, fluorescence kashewa, OJIP sauri fluorescence amsa curve, QA re-oxidation, da dai sauransu, samun fiye da 70 sigogi da su hoto zane-zane

2) Kayan aiki tare da 10x, 20x, 40x, 63x da 100x keɓaɓɓun biofluorescent abubuwan tunani, za a iya lura da chlorophyll da kuma fluorescence fitar da shi a fili

3) Ƙungiyoyin hasken haske masu motsawa sun haɗa da infrared, ja, shuɗi, kore, farin haske, ultraviolet da nisan ja, da dai sauransu, suna iya nazarin kowane kwayoyin launi ko ƙungiyoyin gashi a cikin shuka / algae

4) Ana iya yin nazarin hoto na GFP, DAPI, DiBAC4, SYTOX, CTC da sauran furotin da launi mai launi

5) High ƙuduri spectrometer iya zurfin bincike daban-daban fluorescence spectrography.

6) Tsarin sarrafa zafin jiki na iya tabbatar da gwajin samfurin gwaji a cikin yanayin zafin jiki daidai, inganta daidaito na gwaji, kuma ana iya gudanar da bincike mai zafin jiki / ƙananan zafin jiki.

Ka'idodin photosynthesis yayin rarrabuwa na exocytes na algae na kifi (Ferimazova, 2014)

4. TsarinHigh spectrum imaging fasaha, bayar da shawarar IQ high spectrum imager, All-in-zane, gina atomatik tsufa, data tattara sarrafawa, tsarin aiki, taɓa allon da kuma aiki key, GPS da sauransu, jiki karami da haske, kawai 1.3kg, cimma sauki hannu aiki ko tashi aiki, bandwidth kewayon 400-1000nm, 7nm spectrum ƙuduri, 204 bandwidth, hoton ƙuduri 512x512 pixels, hangen nesa 31 digiri, abu nesa 15cm zuwa infinite nesa, 1m hangen nesa 55x55cm. Zaɓi tare da sauran bincike high spectrum imaging nazarin unit (duba kasa high spectrum imaging fasaha zaɓi reference tebur)

5. Ana iya zaɓar binciken hoto na RGB ko binciken hoto mai yawa a lokaci guda

6. Flexible, sauki don amfani da, zaɓi tare da daban-daban raka'a combination

Binciken hoto na spectrum na algae mai kore, brown algae da ja algae (Ginneken V, 2017)